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Mitegen Llc 24-well sitting drop/hanging drop pregreased crystallization plates
Visualization of the soaking experiment. The lysozyme crystals before staining (left) were grown under the following conditions: 60 mg·mL −1 of lysozyme in 50 m m sodium acetate pH 4.6, 30% w/v PEG 6000 and 0.25 m NaCl (2 μL : 2 μL) after 30 min at 20 °C were visualized under the light microscope. The same <t>crystallization</t> droplet after the application of a crystallization dye (right) and an incubation of 30 min. The dye enhances the contrast and makes the crystals appear prominently colored.
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Visualization of the soaking experiment. The lysozyme crystals before staining (left) were grown under the following conditions: 60 mg·mL −1 of lysozyme in 50 m m sodium acetate pH 4.6, 30% w/v PEG 6000 and 0.25 m NaCl (2 μL : 2 μL) after 30 min at 20 °C were visualized under the light microscope. The same crystallization droplet after the application of a crystallization dye (right) and an incubation of 30 min. The dye enhances the contrast and makes the crystals appear prominently colored.

Journal: FEBS Open Bio

Article Title: Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking

doi: 10.1002/2211-5463.13913

Figure Lengend Snippet: Visualization of the soaking experiment. The lysozyme crystals before staining (left) were grown under the following conditions: 60 mg·mL −1 of lysozyme in 50 m m sodium acetate pH 4.6, 30% w/v PEG 6000 and 0.25 m NaCl (2 μL : 2 μL) after 30 min at 20 °C were visualized under the light microscope. The same crystallization droplet after the application of a crystallization dye (right) and an incubation of 30 min. The dye enhances the contrast and makes the crystals appear prominently colored.

Article Snippet: 24‐well sitting drop/hanging drop pregreased crystallization plates (MiTeGen, Jena, Germany).

Techniques: Staining, Light Microscopy, Crystallization Assay, Incubation